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    This position is located in the Cell Systems Science Group, B&B Division, MML in Gaithersburg, Maryland.

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    Your application package may be shared with other selecting officials at NIST with opportunities like the one you are applying to. Additional selections may be made through this vacancy. 

    This notice is issued under direct-hire authority to recruit new talent to occupations for which NIST has a severe shortage of candidates.

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    The incumbent will support the NIST program in engineering biology by developing engineered cell lines and performing quantitative and automated imaging of living cell populations.  This purpose of the project is to assess the probabilistic response of several genes involved in controlling pluripotency by quantifying their covariance in expression using live cell microscopy. The incumbent will design a CRISPR-based engineering protocol to modify induced pluripotent stem cells such that they faithfully synthesize fluorescent proteins in response to activation of genes of interest; this work involves the use of commercial software to design plasmids and guide RNA of appropriate sequence to result in the desired cellular outcome; Mammalian cell lines will be transfected, isolated and expanded; and quality of the produced lines will be assessed by genomic and functional methods.  This effort requires documentation of the software tools, reagents and performance metrics of the products.  The incumbent will study cells using automated microscopy which requires knowledge of the operation of the fluorescent microscope and environmental control system, use of benchmarking materials to assure image quality, and documentation of experimental details and image data handling requires familiarity with NIST-developed and commercial software.  This work is to be undertaken in close collaboration with image analysis experts and theorists. 

    Travel Required

    Not required

    Supervisory status


    Promotion Potential


This job originated on www.usajobs.gov. For the full announcement and to apply, visit www.usajobs.gov/GetJob/ViewDetails/518175100. Only resumes submitted according to the instructions on the job announcement listed at www.usajobs.gov will be considered.