About the Agency
The position is located at the National Wildlife Research Center (NWRC) in Fort Collins, CO. The coordination of the employee’s activities will be the responsibility of the Project Leader for the Management of Ungulate Disease and Damage Project (MUDD).
This is an excepted service term appointment not to exceed 2 years. (Appointment may be extended up to 4 years without re-announcing.) For more details click: Term Appointments
APHIS is an emergency response agency. This means that all Agency employees may be asked or assigned to participate in rotating temporary duty assignments to support emergency programs at some time during their careers with APHIS. For details click: APHIS Emergency Response
The duties may include, but are not limited to:
- The incumbent will have major involvement with animal studies designed to address: The capability of breath and fecal VOC analysis to allow discrimination between healthy, Mycobacterium bovis (bovine tuberculosis) infected, BCG vaccinated, and M. avium paratuberculosis (MAP) infected cattle; the potential for fecal VOC analysis to detect disease (bovine tuberculosis) in wildlife (white-tailed deer; WTD).
- Responsibilities will include collaboration with Wildlife Services Operations, National Wildlife Research Center, USDA-APHIS-Animal Research Service, National Animal Disease Center, and university scientists and biologists designing and completing studies, collating and analyzing data sets, and preparing draft reports and manuscripts for presentation and publication
- Responsibilities will include planning, coordinating, designing, and implementing animal and laboratory studies that address the research areas outlined above.
- The incumbent will perform a variety of technical procedures on wildlife, including animal capture, restraint, marking, transport; collection of biological data and samples; and chemical immobilization; microbiological isolations and identifications
- Incumbent will comply with Good Laboratory Practices, Institutional Animal Care and Use Committee, Animal Welfare Act, Endangered Species Act, National Environmental Policy Act, and applicable state and federal wildlife laws and regulations.
- The incumbent will be expected to be proficient with a variety of laboratory procedures including basic laboratory safety, work with BSL-2 and BSL-3 pathogens, basic laboratory equipment, ELISAs, PCR, light microscopy, and sample preparation/handling for gas chromatography-mass spectrometry (GCMS).
- Develop and implement animal studies designed to collect data on biological endpoints, including but not restricted to: handling of domestic and wild animals, breath and fecal samples, GCMS analysis, gavage, swab sample collection, blood collection, immobilization, euthanasia, tissue collection and appropriate preservation, and necropsy.
- Perform a variety of technical laboratory procedures on biological specimens, including but not restricted to: isolation and identification of viral and bacterial pathogens, polymerase chain reactions (PCR), reverse transcriptase-PCR (RT-PCR), Enzyme-linked immunosorbent assays (ELISA), pulse field gel electrophoresis (PFGE), cell culture methods, other serological methods.
- Operate, and maintain basic and advanced laboratory equipment: e.g., pH meters, balances, centrifuges, ELISA plate washers and readers, RNA/DNA tissue processors, spectrophotometers, incubators, CO2 incubators, thermocyclers, real time PCR systems, robotic nucleic acid extraction systems, PFGE systems, light and fluorescent microscopes, GCMS systems.
- Perform other related duties as assigned.
- Occasional Travel
- Travel may be required.