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This position is located in the Vaccine Research Center (VRC), National Institute of Allergy and Infectious Diseases (NIAID). The Vaccine Research Center (VRC) conducts research that facilitates the development of effective vaccines for human disease with a primary focus on developing vaccines for AIDS. Activities at the VRC include 1) basic research to establish mechanisms for inducing long-lasting protective immunity against HIV and other pathogens that present special challenges to vaccine development; 2) the conception, design, and preparation of vaccine candidates for HIV and other viruses; and 3) laboratory analysis, animal testing, and clinical trials of such candidates.
If selected for this position, you will perform the following duties:
- Lead activities for the stable cell line development of CHO, HEK293 and other mammalian cell lines in the Vaccine Production Program Laboratory(VPPL).
- Participate in collaborations with other intramural and extramural investigators to develop banks of stable research cell lines for rapid cell line generation.
- Investigate and develop systems for recombinase mediated cassette exchange systems as well as standardize methodologies for generation of stable cell lines using the chinese hamster ovary (CHO)-glutamine synthetase (GS) system, CHO-dihydrofolate reductase (DHFR) amplification system and other methods.
- Keep abreast of pertinent literature and incorporate appropriate concepts and techniques into research activities and experiments.
- Identify, define and/or select specific problems for study.
- Evaluate progress of experiments to ensure that they are proceeding as planned.
- Independently interpret and evaluate experimental data.